Actividad de la telomerasa e infección por VPH en raspados cervicales de mujeres que desarrollaron lesiones escamosas intraepiteliales de alto grado. Estructura dimérica de la telomerasa. LA TELOMERASA Chloé Larrue, Anna Lippert, Carmen Majano, Héctor Peláez y Alicia Santos. Request PDF on ResearchGate | Actividad de la telomerasa y Article in Revista espanola de enfermedades digestivas: organo oficial de la.
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Fill out the form below to receive a free trial or learn more about access:. Quantitative analysis of telomerase activity: Six of these patients had colonic tumors, polyps, and macroscopically normal mucosa 10 cm away from the tumor, while the remaining 8 samples had polyps and normal mucosa. In 6 of these patients fresh samples of tumor tissue, polyps, and normal mucosa were obtained; in the 8 remaining cases, we collected only polyps and normal mucosa.
This part of the study could not be compared with other similar studies as we found none the literature. Results The present pilot study includes 14 patients with colorectal polyps, of which 6 showed synchronous colorectal polyps and cancer, while the remaining 8 were isolated polyps.
Mean polyp telomere length was 6. The presence of synchronous cancer modifies polyp telomerase activity.
Currently, there is clinical, epidemiological and experimental evidence clearly indicating that polyps or adenomas are precursor lesions for most colorectal malignancies.
Mean TA in polyps was 5.
The present pilot study includes 14 patients with colorectal polyps, of which 6 showed synchronous colorectal polyps and cancer, while the remaining 8 were isolated polyps.
Polyps synchronic with tumors have a telomerawa lower telomerase activity when compared to isolated polyps. We think that with more cases those differences could be significant and confirm the existence of a sequence in telomere behavior TA and TL from normal towards tumoral mucosa through polyps.
La longitud del telómero y la actividad de la telomerasa, una Yin y Yang de la senescencia celular
This finding would be a mechanism taking into account the changes telomreasa known to occur in this sequence, including deletions, mutations, oncogene activation, suppressor genes, or loss of heterozygosity. We also studied the normal – adenoma – carcinoma sequence by measuring TA and TL in samples from 6 patients who had synchronous polyps and colorectal cancer, in which we also analyzed the normal mucosa.
Centrifugar la muestra durante cinco minutos a la misma velocidad y temperatura para volver l pellet de la muestra.
telomerass Polyps in patients without synchronous cancer had a telomerase activity that was significantly higher 9. Pathology of colorectal cancer. In the adenoma-cancer sequence we have demonstrated that there is obvious telomeric dysfunction.
In vitro Reconstitution of the Active T. The unit of telomere length was Kilo-base pairs Kbp. Average telomere length in tumors was 6. Examination of the Telomere G-overhang Structure in Trypanosoma brucei. Telomerase as tumor marker. This behavior we think it due to the fact that the cell division ratio also increases with the passage from one tissue type to the next. Mean TA in tumor samples was 5. Cristina Valls is supported by a predoctoral grant by Generalitat de Catalunya. Centrifugar el homogeneizado a On the other hand, it allows to study the sequence in each patient individually.
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Actividad de la telomerasa y longitud del telómero en la secuencia pólipo-carcinoma colorrectal
Skip to content Biology. Hepatogastroenterology ; 48 Other authors published polyp TA figures that oscillate between 1. The objective of this study was to determine telomerase activity and telomere length patterns in patients with adenomatous polyps either associated or not with colorectal lla, as well as the role of telomeric instability in the adenoma-carcinoma sequence.
Girar la muestra a Departments of 3 Gastroenterology and 4 Surgery. Dis Colon Rectum ; 50 5: Am J Gastroenterol ; 96 4: Toxicol Pathol ; 27 6: Int J Oncol ; 20 1: This progressive shortening is an important mechanism in the timing of human cellular aging.
Skip to content Biology. When these cells accumulate sufficient mutations affecting the cell cycle, they can become telomeraaa. Alternative lengthening of telomeres ALT and chromatin: You must be signed in to post a comment.
Demonstration of human telomerase reverse transcriptase in human colorectal carcinoma by in situ hybridization. Paradoxically, those results are accompanied by greater telomere length in the former. Telomerase activity is a prognostic factor for recurrence and survival in rectal cancer. Telomerase activity in tumors was 5.
Structure and function of telomeres.
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